BioZone is a focal point for bioengineering research at the University of Toronto.
Our facilities provide a collaborative space and cross-disciplinary approach that enable researchers to share knowledge, processes and equipment as they tackle difficult technical problems in environment, energy and medicine. The facility occupies the west wing of the 3rd and 4th floors of the Wallberg building at the University of Toronto, providing over 1,800 m2 of laboratory and research workspace, including several large, bright, collaborative research labs.
BioZone research concerns enzymes, cells and organisms that can be harnessed for the creation of renewable bioproducts, fuel cells and medical therapies, and for the remediation of soil, water, and air. Computational approaches to screen and identify novel metabolic pathways and to engineer cellular processes play a central role in BioZone. Novel cultivation techniques, from microbial to human cells, are used to develop valuable new products.
The facility’s research labs house a wide array of analytical instruments for molecular biology, protein purification and identification, enzyme kinetics, substrate and metabolite analysis, microscopy and cell growth. The diversity of research within BioZone provides exposure to a wide range of expertise and analyses that can be useful for any given project, arming students with a broad array of state-of-the art techniques to tackle complex problems.
- Exactive MS coupled to Accela 1250 pump and autosampler
- LTQ XL with EASY nLC / Proxeon
- Q Exactive (LC-MS)
- Varian Saturn 2100T GC/MS
- Mass Spec Facility Website
- Infors HT Minifors (5L)
- Chemglass bioreactor (20L) , suitable for hydrolysis, fermentations or any other type of aqueous phase reaction at temperatures up to about 70ºC. Used to explore hydrolysis of biomass at high solids – 15 to 25%.
- Bench Scale Biofiltration System: continuous parallel bench scale bioreactors (app. 5 ft x 1 ft) for the study of biological gas cleaning. Their large size is suitable for testing various packing media. Gas and media sampling is possible for chemical, physical and microbial analysis. The system allows for introducing simulated air emissions with various air pollutants at controlled temperatures.
- Reduced Sulfur Biofiltration System: continuous biofiltration units for the study of the treatment of reduced sulphur pollutatnts such as hydrogen sulphide and dimethyl sulphide.
1. Rigaku MICROMAX-007 x-ray generator with Rigaku R-axis 4++ image plate detector
2. Rigaku MICROMAX-007 x-ray generator with Rigaku HTC image plate detector
1. OptimaTM L-90K ultracentrifuge (Beckman Coulter)
2. Avanti J-E high speed centrifuge (Beckman Coulter)
3. Allegra centrifuge including a swinging bucket rotor and plate adapter (Beckman Coulter)
4. Biofuge fresco microcentrifuge (Sorvall)
5. DNA 120-115 speed vac (Thermo Electron Corp)
6. Avanti J-E 251 high performance centrifuges (Beckman Coulter)
Laminar flow hoods provide a contaminant-free environment for work with biological samples. They are equipped with High Efficiency Particular Air (HEPA) Filters that remove any microorganisms from the air before it gently flows through the hood in laminar fashion.
- Olympus CKX1 live cell inverted microscope
- Olympus 120Q live cell inverted microscope
- Olympus BX51 microscope
- Leitz Laborlux S microscope
- Molecular Devices Image Xpress Micro Widefield High Content Screening System
Examples of current projects in the BioZone that utilize the microscope facility include, quantifying the alignment re- sponse of airway epithelial cells cultured on substrates contain- ing guidance signals, characterizing the cooperative behaviour of epithelial cells re-organizing in confluent 2D monolayer sheets, assessing cellular invasion of cancer cells into sur- rounding stromal tissues in 3D, and real-time monitoring of engineered tumor growth in response to drug treatments.
- AM Harrick Plasma