Synthesis of proteins from genome and metagenome sequences, high throughput screening of their potential enzymatic activities, biochemical characterization of novel enzymes, crystallization and structural analysis using X-ray crystallography. Characterization of hydrolytic enzymes and enzymes for biopolymer synthesis.

Links to relevant research groups and projects:

• Structural Microbiology laboratory
• Bioprocess and Enzyme Technology Lab

 

 

Modeling and analysis across multiple time and length scales with a focus on metabolic and gene regulatory networks.

Links to relevant research groups and projects:

• Laboratory for Metabolic Systems Engineering

 

 

Maximizing growth of microalgae from carbon dioxide, sunlight and wastewater for production of biofuels and biochemicals.

Links to relevant research groups and projects:

• Bioprocess Engineering Lab

 

 

Bioproducts, i.e., products manufactured from renewable resources or using biological production systems, are the foundation of the new global low carbon bioeconomy. This bioeconomy will support many sectors including commodity chemicals and materials, health, agriculture, and energy. BioZone researchers are developing biotechnologies (enzymes and cells) that can convert renewable feedstocks such as agricultural waste into chemicals, bioplastics, and other products.

Links to specific research groups and projects:

• Industrial Biocatalysis Network
• Laboratory for Metabolic Systems Engineering
• Bioprocess Engineering Lab
• Bioproducts Research Lab
• Bioprocess and Enzyme Technology Lab

Biological treatment systems for wastewater and air emissions. Biological conversion of wastes from forestry and other industries into renewable fuels, value added chemicals, and renewable materials.  Investigation of enzymes that degrade lignocellulose and their expression in plants.  Enzymatic hydrolysis of starchy and lignocellulosic substrates.

Links to specific research groups and projects:

• Bioprocess Engineering Lab
• Bioproducts Research Lab
• Bioprocess and Enzyme Technology Lab
• University of Toronto Pulp and Paper Centre

Strategies for reactor scale-up, processing of high consistency slurries, viscosity and inhibitor management.

Links to relevant research groups and projects:

 

• Bioprocess and Enzyme Technology Lab
• Laboratory for Metabolic Systems Engineering

 

Comprehensive evaluation of capital and operating costs of biofuel production. Economic impact of high value co-products. Greenhouse gas and critical air contaminants impacts of biofuels, bioenergy and bioproducts. Bioenergy policy.

Links to relevant research groups and projects:

• Bioprocess and Enzyme Technology Lab

 

 

 

Biological reductive dechlorination of chlorinated solvents, including chlorinated ethenes, ethanes, and methanes. Zero-valent iron reduction of chlorinated ethenes. Biodegradation of benzene, toluene, ethylbenzene, and xylenes under various electron-accepting conditions. Biological reduction of metals. Characterization of anaerobic mixed microbial communities and their enzymatic processes, including genomic and metagenomic analyses, metabolic network reconstruction and modeling.

Links to relevant research groups and projects:

• Laboratory for Metabolic Systems Engineering
• Biodegraders Research Group

 

 

Molecular biological tools for reductive dechlorination, metal reduction, wastewater treatment granules and flocs.  Laboratory techniques include polymerase chain reaction (PCR), quantitative PCR, reverse transcriptase PCR (RT-PCR), microarrays, next-generation sequencing, protein gel electrophoresis, and native protein polyacrylamide gel electrophoresis (PAGE).

Links to relevant research groups and projects:

• Bioproducts Research Lab
• Bioprocess Engineering Lab
• Biodegraders Research Group

Anaerobic digestion of organic waste has tremendous potential to address the economic and the environmental pressures facing most industries and municipalities. While anaerobic digestion is widely applied already in certain sectors, there is keen interest to expand its use to new waste streams, for example in the pulp and paper industry. Using a combination of pre-treatment methods and reactor configurations, combined with new molecular and analytical tools to gain mechanistic information on anaerobic processes, we are investigating alternative approaches to recover energy from waste liquid and solid streams.

Links to specific research groups and projects:

• Biodegraders Research Group
• Bioprocess Engineering Group

Antibiotic resistance is a serious and growing health concern. Due to misuse or overuse of antibiotics, bacteria have evolved various mechanisms to reduce the effectiveness of these drugs. Many clinically-relevant species, including the ESKAPE pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species contain either plasmids or chromosomal genes that produce enzymes with activities rendering antibiotics inactive.

The Structural Microbiology Lab is carrying out structure-function and inhibition studies of enzymes conferring antibiotic resistance to the glycopeptide drug of last resort vancomycin, aminoglycosides, streptogramin compounds and other antibiotics. We use X-ray crystallography as our primary tool to produce detailed insights into molecular structure of these enzymes and use this information to guide design of potent inhibitory compounds that can be used as antibiotic adjuvants for combined antimicrobial therapy.

Links to specific research groups and projects:

• Structural Microbiology Lab

Microfabrication tools for biology, developmental biology, systems biology, cell organization and self-assembly, cell migration, patterned gene delivery, and modelling tissue organization mechanisms.

Links to specific research groups and projects:

• McGuigan Lab for Tissue Morphogenesis Engineering

Microencapsulation of food ingredients and nutraceuticals for controlled release of micronutrients in food fortification – both for international development, and for the North American food industry.

Links to specific research groups and projects:

• Food Engineering Group
• Micronutrient Initiative